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- 2025年07月11日
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文献和实验DNA Fragment Purification from Agaro
DNA Fragment Purification from Agarose or Acrylamide For fragments from 200 bp to 10 kb the agarose purification is ideal.For smaller fragments (20 bp to 400 bp)the acrylamide purification is preferred. Solutions Crush and Soak Solution 500 mM
TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve) 2.Frozen agarose gel piece containing the desired DNA fragment Supplies: 1.Micropipetter and tips 2.Microcentrifuge and tubes 3.Spatula 4.PCR machine and the 600-ul
Abstract Functional genomics require manipulation and modification of large fragments of the genome. Such manipulation has only recently become more efficient due to the discovery of different techniques based on homologous
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