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- 2025年07月12日
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文献和实验Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
a standard alkaline phosphatase staining kit to reveal expression of the pluripotent stem cell marker APLP. Note that while hCD29 is expressed by all human pluripotent stem cells throughout each colony (E), this antibody fails to stain live cells
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Spring Harbor, NY, USA, 2007. INTRODUCTION In the protocol presented here, hematopoietic stem cells (HSCs) are specifically transduced with a vector displaying the HSC-activating polypeptides, stem cell factor (SCF) and thrombopoietin (TPO
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
a useful approach to preparing highly reproducible samples of human embryonic stem cell (hESC) total RNA suitable for transcriptional profiling from heterogeneous mixtures of cells containing undifferentiated hESC and differentiated cell types. In this unit
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