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- 2025年07月13日
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100 ul
| Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| W | 1:1000 | Human, Mouse, Rat | Endogenous | 60 | Rabbit IgG |
| IP | 1:200 | ||||
| IF-IC | 1:200 | ||||
| F | 1:400 |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Protocols
4058:
- Western Blotting, Immunoprecipitation, Immunofluorescence, Flow
Specificity / Sensitivity
Phospho-Akt (Ser473) (193H12) Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Ser473.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt.
Western Blotting
Western blot analysis of extracts from untreated or PDGF-treated NIH/3T3 cells, pretreated with wortmannin #9951 and/or rapamycin #9904 as indicated, using Phospho-Akt (Ser473) (193H12) Rabbit mAb (upper) or Akt Antibody #9272 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 #9901 and Wortmannin #9951 treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of C2C12 cells, U0126/LY294002/Rapamycin-treated (left) or insulin-treated (right) using Phospho-Akt (Ser473) (193H12) Rabbit mAb (green). Actin filaments were labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye)
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