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文献和实验Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown
expressions are regulated. Commonly used techniques to determine DNA-protein binding such as the electrophoretic mobility shift assay (EMSA) have limited value for analyzing simultaneously a large number of proteins in the complex
lution of GST-Fusion Protein from Glutathione Agarose
lution of GST-Fusion Protein from Glutathione Agarose 1. Wash the GST-fusion protein glutathione-agarose complex once with 1% Triton X-100 in PBS. 2. Resuspend the complex in 1% Triton X-100 in PBS. 3. Prepare a small column
Agarose Gel Electrophoresis of RNA
实验试剂 Gel Loading Buffer II Gel Loading Solution NorthernMax Formaldehyde Load Dye NorthernMax-Gly Sample Loading Dye 实验步骤 1. Prepare the gel. 1) Heat 1 g agarose in 72 ml
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