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- 高度特异性的miRNA Hairpin primer。
- 高特异性Taqman-MGB探针，保证灵敏度的同时高度特异。
- 只对成熟MicroRNAs进行定量，不受其前体干扰；序列高度同源的miRNAs也可精确区分。
- 灵敏度极高，仅需1-10ng的total RNA或同等物。
- 超宽的定量线性范围，可跨越7个数量级，从几个拷贝一直到几百万个拷贝。
- 可实现对每个细胞所含目标miRNA分子进行准确定量。
- 试剂盒内含合成的miRNA标准品一支。
- 试剂盒包含优化高效的逆转录试剂。
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文献和实验相关实验
impossible to obtain with SYBR Green, since they will only hybridize to true targets in a PCR and not to primer-dimers or other spurious products. Quantitation of Results Two strategies are commonly employed to quantify the results obtained by real-time RT
Real-Time PCR Quantification Using Cloned Standards and Multiple Housekeeping Genes
with this technique, is implemented in our facility and can be easily adopted by any laboratory. This procedure is based upon SYBR green detection, though many of the quantification algorithms can be used with fluorescent probe technologies. There are two methods
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Two Step Stemaim-it miR qRT-PCR Quantitation Kit(SYBR Green)/探针法miRNA检测试剂盒 8.5折扣优惠
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