CYCLIN B MAB

【求助】A与B结合抑制了B与C的结合能说竞争性抑制吗

！ 我的问题说起来稍微有点复杂！前期试验已经证实，A与B在体外及细胞内结合，A过表达能够抑制作为蛋白激酶的B的激酶活性，同时我们检测到了A过表达在蛋白水平抑制了B与C的表达（B的下降幅度远大于C），B是一种目前研究得比较透彻的细胞周期依赖性蛋白激酶CDK，而C呢也是公认的与B结合的cyclin。目前我们已经初步得到了A降低B与C表达水平的原因是通过A抑制B与C表达的上游基因导致的，但是作为B这样的CDK，A与之结合是一定要对其产生一些什么作用的。于是我们研究了A对B的降解，磷酸化调节等过

Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reaction

Disorders of the cell cycle regulatory machinery play a key role in the pathogenesis of cancer. The gene encoding the cyclin D1 protein, a regulator of the progression from the G1- to S-phase, is often found disrupted in the cancer cell

Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）

the need for fluorescence compensation. This assay can be used with any instrument employing dual laser excitation, including the B-D Vantage, FACSCalibur and Coulter Elite. Materials Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326) or cyclin