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- 2026年02月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
6个月
- 英文名：
ATP Content HPLC Assay Kit
- 库存：
大量
- 供应商：
Solarbio
- CAS号：
BC0304-50T/48S
- 保存条件：
-20℃
- 规格：
50管/48样
产品简介
| 中文名称 | ATP含量检测试剂盒 |
| 储存条件 | -20℃ |
| 有效期 | 6个月 |
| 英文名称 | ATP Content HPLC Assay Kit |
| 检测方法 | 高效液相色谱法 HPLC |
| 单位 | 盒 |
| 规格 | 50T/48S |
注意：正式测定之前选择 2-3 个预期差异大的样本做预测定。
产品简介： 三磷酸腺苷（adenosine triphosphate, ATP）被认为是一种在所有生物体生存和繁殖的细胞合成中 必不可少的普遍能量来源。ATP 可通过多种细胞途径产生。最典型的如在线粒体中通过氧化磷酸化 由三磷酸腺苷合酶合成，或者在植物的叶绿体中通过光合作用合成。ATP 合成的主要能源为葡萄糖 和脂肪酸。 ATP 在 254 nm 下有吸收峰，可以利用高效液相色谱法测定其含量。
试验中所需的仪器和试剂： 高效液相色谱仪（C18 柱（4.6×250 mm），紫外检测器（VWD））、台式离心机、可调式移液 枪、研钵/匀浆器、棕色 EP 管、针头式过滤器（50 个，水系，0.45 µm），注射器，抽滤器，滤膜 （有机系、水系），棕色进样瓶（50 个，2 mL）、（色谱纯，500 mL）、超纯水。
产品内容：
提取液一：液体 80 mL ×1 瓶，4℃保存。
提取液二：液体 40 mL ×1 瓶，4℃保存。
试剂一：液体 15 mL ×1 瓶，4℃保存。临用前取 3.5 mL 试剂一加入到 1000 mL 超纯水中，用试 剂二调节其 pH = 6.15，形成流动相 B，密封。
试剂二：液体 5 mL ×1 瓶，4℃保存。
标准品：粉剂×1 支，-20℃避光保存。临用前加入 1.65 mL 蒸馏水配制成 1 μmol/mL ATP-Na2 标准溶液，-20℃冻存。为了保证 ATP 的完整性，请避免反复冻融。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
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4.5000
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Microorganisms
PMID：
38138019
Transcriptomic and metabolomic analysis of the mechanism by which Bacillus tequilensis inhibits Alternaria alternata to control pear black spot
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Author：
Qiuyan Bi, Pengfei Liu, Jie Wu, Fen Lu, Xiuying Han, Wenqiao Wang, Jianjiang Zhao
IF：
4.2000
Publish_to：
BIOLOGICAL CONTROL
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Author：
Limin Wang, Xiaoyu Song, Yi-nan Cheng, Senxiang Cheng, Tong Chen, Honglian Li, Jingming Yan, Xiafei Wang, Haifeng Zhou
IF：
4.1000
Publish_to：
PEST MANAGEMENT SCIENCE
PMID：
38010196
Network Pharmacology and Molecular Docking Explore the Mechanism of Mubiezi-Yinyanghuo Herb Pair in the Treatment of Rheumatoid Arthritis
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Author：
Fuxue Meng, Xiaomai Tao, Longkuan Li, Wei Jia, Xin Yang, Yuchen Yang
IF：
2.6500
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Evidence-based Complementary and Alternative Medicine
PMID：
38106514
Circular RNA hsa_circ_0000175 Serves as a Potential Biomarker for Rheumatoid Arthritis via miR-31-5p/GSDME Axis
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Author：
Xin Panpan, Tan Zhiming, Wang Zhiwen, Chen Yuhang, Zhuang Yu
IF：
2.4000
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BIOCHEMICAL GENETICS
PMID：
37968534
"Antifungal effects of carvacrol
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Author：
the main volatile compound in Origanum vulgare L. essential oil
IF：
0.0000
Publish_to：
Shuai-Bing Zhang
PMID：
Yang-Yon
相关实验
还有就是用Invitrogen公司的CFSE细胞增殖流式检测试剂盒也可，我觉得。 qing0831 可以用流式细胞术检测，我经常做这方面的实验，可以交流一下，手机：15921609870 QQ：57325595 bigbang_0_0 cologenic assay bigbang_0_0 cologenic assay nmmxq Brdu incorporation assay
，当时是用的是PE公司的检测试剂盒，效果还不错。记得做的时候是需要加IBMX的，好像是抑制cAMP分解的 wlily sunopal_5200 wrote: 请问您做出来了吗？我也买了RD试剂盒，预实验都不理想，值太低。楼主做时加IBMX了吗？ 做得怎么样了？我最近也在做这个，做了三次，标准曲线两次做得不错，但样品浓度有差异 biodiagnosis 2010年用过
为ATP，随后能利用ATP发光的luciferase酶就可以指示支原体的存在。 03 ELISA检测法 ELISA也能用于支原体检测，以ELISA法为基础的支原体检测一般使用针对支原体16S rRNA基因的带标记探针或抗体，来检测培养物中是否含有支原体。 04 DNA荧光染色法 需要将可疑样品与指示细胞共同培养，因此一般需要几天时间。DNA检测所用的指示细胞通常是细胞质区域较大的Vero细胞，如果原样本中含有支原体，那么当细胞DNA被荧
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ATP含量测定检测试剂盒
￥620
