Dynabeads® CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45+ leucocytes directly from whole blood, buffy coat, or MNC suspensions. Dynabeads® CD45 can also be used to enrich epithelial tumor cells. The CD45 antigen is weakly expressed on myeloid cells. To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads® CD15 (Cat. No. 11137D) in combination with Dynabeads® CD45. Advantages of Dynabeads® CD45:
• Efficient depletion of human leucocytes • Isolation of leucocytes directly from whole blood for molecular applications • Enrichment of circulating epithelial tumor cells
About Dynabeads® CD45 Dynabeads® CD45 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal mouse IgG2a antibody specific for a CD45 membrane antigen common to all known isoforms of CD45. CD45 is expressed on all human leucocytes. Because of the bead size,
Buffy coat, also known as leukocyte concentrate, is the middle fraction of an anti-coagulated blood sample that sits under the plasma and on the top of red blood cells after centrifugation of the sample without using a density gradient reagent such as Ficoll solution. Buffy coat contains both leukocytes and platelets and can be used as a source of this cellular material.