Toll样受体信号通路PCR芯片Toll-Like Rece

Toll样受体（TLR）信号通路PCR芯片可以同时检测84个在TLR介导的信号转导和先天免疫中发挥关键作用的基因。TLR家族的模式识别受体（PRRs）能够通过病原体关联的分子模式检测细菌，病毒，真菌和寄生虫。每个受体结合特异性的配体，进而起始针对特定病原体的先天免疫反应，并激活获得性免疫反应。例如，TLR4能够识别细菌脂多糖（LPS）或者是在血源性感染中引起感染性休克的内毒素。这些受体通过单体或异质二聚体的形式与受体蛋白相互作用，而从激发MyD88或TICAM1（TRIF）依赖的免疫反应。这些反应主要是通过NFκB分子激发信号级联反应。NFκB能够激活下游的JNK/p38信号或细胞因子分泌。这些信号通路的失活会导致严重的后果，并且引起多种自身免疫性疾病和病理性的炎症。这款芯片包含了TLR信号分子家族、受体、效应分子、以及受TLR激活的下游信号通路中的关键基因。这些下游信号通路包括：NFκB、JNK/p38、IRF 和JAK/STAT信号通路。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测TLR介导的信号转导相关基因的表达。

Product	Species	Technology	Cat. No.
Toll-Like Receptor Signaling Pathway PCR Array	Human	Gene Expression	PAHS-018Z
Toll-Like Receptor Signaling Pathway PCR Array	Mouse	Gene Expression	PAMM-018Z
Toll-Like Receptor Signaling Pathway PCR Array	Rat	Gene Expression	PARN-018Z
Toll-Like Receptor Signaling Pathway PCR Array	Rhesus Macaque	Gene Expression	PAQQ-018Z

Toll-Like Receptors:CD180 (LY64), SIGIRR, TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10. Pathogen-Specific Responses: Bacterial: CCL2 (MCP-1), CD14, CD180 (LY64), FOS, HRAS, IL10, IL12A, IL1B, IL6, IL8, IRAK1, HMGB1, HSPA1A (HSP70 1A), JUN, LTA (TNFB), LY86 (MD-1), LY96, NFKBIA (IKBA/MAD3), PTGS2 (COX2), RELA, RIPK2, TLR2, TLR4, TLR6, TNFRSF1A, TICAM1 (TRIF). Viral: EIF2AK2 (PRKR), IFNB1, IFNG, IL12A, IL6, IRF3, PRKRA, RELA, TBK1, TLR3, TLR7, TLR8, TNF, TICAM1 (TRIF). Fungal/Parasitic: CLEC4E, HRAS, HSPA1A (HSP70 1A), IL8, TLR2, TIRAP. TLR Signaling: Negative Regulation:SARM1, SIGIRR, TOLLIP. TICAM1 (TRIF)-Dependent (MYD88-Independent): IRF3, MAP3K7 (TAK1), TAB1, NR2C2, PELI1, TBK1, TICAM2, TLR3, TLR4, TRAF6, TICAM1 (TRIF). MYD88-Dependent: IRAK1, IRAK2, MAP3K7 (TAK1), TAB1, MYD88, NR2C2, TIRAP, TLR1, TLR10, TLR2, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TRAF6. Downstream Pathways and Target Genes: NF?B Pathway:BTK, CASP8, CHUK (IKKa), ECSIT (SITPEC), FADD, IKBKB, IL10, IL1B, IRAK1, IRAK2, IRF3, LY96, MAP3K1 (MEKK), MAP3K7, MAP4K4, NFKB1, NFKB2, NFKBIA (IKBA/MAD3), NFKBIL1, NFRKB, PPARA, REL, RELA, TNF, TNFRSF1A, UBE2N. JNK/p38 Pathway:ELK1, FOS, IL1B, JUN, MAP2K3 (MEK3), MAP2K4 (JNKK1), MAP3K1 (MEKK), MAP3K7, MAPK8 (JNK1), MAPK8IP3, TNF. JAK/STAT Pathway: CCL2 (MCP-1), CSF2 (GM-CSF), IFNG, IL12A, IL2, IL6. Interferon Regulatory Factor (IRF) Pathway:CXCL10 (INP10), IFNA1, IFNB1, IFNG, IRF1, IRF3, TBK1. Cytokine-Mediated Signaling Pathway: CCL2 (MCP-1), CSF3 (GCSF), IL1A, IL1B, IL6, IRAK1, IRAK2, RELA, SIGIRR, TNF, TNFRSF1A. Regulation of Adaptive Immunity:CD80, CD86, HSPD1, IFNG, IL10, IL12A, IL1B, IL2, MAP3K7, TRAF6. Adaptors & TLR Interacting Proteins:BTK, CD14, HMGB1, HRAS, HSPA1A (HSP70 1A), HSPD1, LY86 (MD-1), LY96 (MD-2), MAPK8IP3, MYD88, PELI1, RIPK2, SARM1, TICAM1 (TRIF), TICAM2 (TRAM), TIRAP, TOLLIP. Effectors:CASP8 (FLICE), EIF2AK2 (PRKR), FADD, IRAK1, IRAK2, IRAK4, MAP3K7 (TAK1), TAB1, NR2C2, PPARA, PRKRA, ECSIT (SITPEC), TRAF6, UBE2N.