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- Polycomb & Trithorax Complexes ChIP PCR Array
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- 2026年01月30日
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- 服务名称：
Polycomb & Trithorax Complexes ChIP PCR Array
- 提供商：
SAB
Human Polycomb & Trithorax Complexes ChIP PCR Array
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| The Human Polycomb & Trithorax Complexes EpiTect Chip qPCR Array profiles the histone modification status or “histone code” of 84 genes encoding key polycomb and trithorax complex components including chromatin modification enzymes and remodeling factors. Histone modifications define chromatin structure, regulate gene activity and specify cellular status. The polycomb and trithorax complexes maintain epigenetic control of cell type specific gene expression patterns important for cellular identity via histone modification. The polycomb complex causes transcriptional repression, while the trithorax complex reverses that effect to maintain an active state of transcription. Dysregulation of these activities promotes oncogenesis by causing inappropriate expression of cell identity, differentiation and proliferation genes. Cancer research studies describe mutations in genes encoding polycomb and trithorax complex components, as well as complex regulators, during oncogenic transformation. Because these complexes play a central role in the epigenetic regulation of oncogenes and tumor suppressor genes, epigenetics may also stabilize their expression patterns, making an analysis of their own "histone code" also essential. This array includes core components of the polycomb and trithorax complexes as well as key interactors and genes necessary for complex assembly. Using chromatin immunoprecipitation and this real-time PCR Array, you can easily and reliably analyze the histone modification patterns associated with a focused gene panel involved in Polycomb and Trithorax complex activity. | ||||||||||||||
Core Components: BMI1 (PCGF4), EED, EZH2, PHC1, PHC2, PHC3, RBBP4, RBBP7, RING1, RNASEL, SCMH1.
Alternate Core Components: CBX5, EZH1, PHF1, RNF2.
Additional Complex Components: CBX8, DNMT1, DNMT3A, DNMT3B, E2F6, KDM2B, KDM5D, PCGF1, PCGF2 (RNF110), PHC2, RING1, RNF2, RNF2, RYBP.
Polycomb Cofactors:
Complex Assembly: AEBP2, RBP2, SNAI1, ZBTB16.
Complex Interacting Genes: ARID1B, ASXL1, ASXL2, ASXL3, BAP1, CBX1, CBX2, CBX3, CBX4, CBX7, CTBP2, DNMT3L, EPC1, EPC2, HTT, INO80 (INOC1), INO80B, INO80C, INO80D, JARID2, L3MBTL2, LARP7, MBTD1, MLL4, MLL5, MOV10, MTF2, PCGF5, PHF19, PPP1CC, PPP1R8, SCML2, SIRT1, SMARCA4, SMARCC1, TRIM27, USP11, USP7, YAF2, YY1AP1, YY2.
Trithorax Complexes:
Core Components: ARID1A, ASH2L, CXXC1, HLTF, MLL2, MLL3, PBRM1, RBBP4, RBBP5, RBBP7, SMARCA1, SMARCA2, SMARCB1, SMARCC2, WDR5.
Complex Interacting Genes: ARID1B, MLL4, SMARCA4, SMARCA5, SMARCC1.
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文献和实验浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
-19]； xChIP：用来研究DNA及低结合力蛋白，采用甲醛或紫外线进行 DNA 和蛋白交联，超声波片段化染色质，然后进行片段富集及后续分析，适用于多数非组蛋白的蛋白，例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后，对 DNA 进行 PCR 扩增，验证目标序列的存在。除验证实验外，ChIP DNA也可以进行测序分析，这种方法被称为 ChIP-seq [22] ；也可做芯片分析，这种方法被称为 ChIP on CHIP 或 ChIP
Chromatin Immunoprecipitation (ChIP)
cross-linking agent for ChIP and the use of polymerase chain reaction (PCR) to detect precipitated DNA fragments were later added as components of the modern ChIP procedure. The protocol below represents a standard ChIP procedure for use in mammalian
染色质免疫沉淀 (ChIP) 用于检测细胞核中天然染色质内的蛋白质与 DNA 之间的相互作用。ChIP 实验首先需要将细胞固定，即将蛋白质与 DNA 相互作用交联固定到位。然后将染色质打断为片段，使用抗体对目的蛋白质以及与其结合的所有 DNA 进行免疫沉淀。最后，解交联，对沉淀 DNA 进行纯化。纯化的 DNA 可进行进一步的分析，如标准或实时 PCR、芯片或测序分析。 这些实验对染色质的完整性、蛋白质表位的质量以及免疫沉淀抗体的特异性非常敏感。尤其当目标蛋白质与 DNA
