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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within human LAMP-1.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 ml IgG in PBS pH 7.4 with 0.02% sodium azide. Antibody was purified by immunogen affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H, M, R
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within human LAMP-1.
- 目录编号:
253569
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
LAMP-1
- 抗体英文名:
LAMP-1 Antibody
- 抗体名:
LAMP-1 Antibody
- 规格:
0.1 mg
LAMP-1 Antibody产品详细介绍:
| 产品名称: | LAMP-1 Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253569 |
| 规格: | 0.1 mg |
| 产品描述: | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within human LAMP-1. |
| 适用物种: | H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名: | LAMP-1; Lysosome associated membrane protein 1 |
| 形态: | Each vial contains 0.1 ml IgG in PBS pH 7.4 with 0.02% sodium azide. Antibody was purified by immunogen affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验LAMP引物设计的在线网站(http://primerexplorer.jp/e/),只要导入靶基因就能自动生成成组引物。 以某一微生物的鞭毛基因为例讲解一下LAMP相物设计的过程: 首先单击浏览钮择靶基因序列文件,靶序列默认的是小于2 2 kbp。支持三个类型的文件,普通文本格式(仅含序列), FASTA格式和GenBank 格式文件。 第二,从下面个项中择定参数设定(引物设计条件条件。基于GC含量的自动判断, 起始的参数是特定的:如果GC含量小于或等于45%.,则选取AT丰度高的区
1.LAMP引物的设计LAMP引物的设计主要是针对靶基因的六个不同的区域,基于靶基因3' 端的F3c、F2c和Flc区以及5' 端的Bl、B2和B3区等6个不同的位点设计4种引物。FIP(Forward Inner Primer):上游内部引物,由F2区和F1C区域组成,F2区与靶基因3’端的F2c区域互补,F1C区与靶基因5' 端的Flc区域序列相同。F3引物:上游外部引物(Forward Outer Primer),由F3区组成,并与靶基因的F3c区域互补。BIP引物:下游内部引物
PCR方法在人类及动植物疾病基因诊断、食品分析和环境监测等领域发挥着举足轻重的作用,其灵敏度高、特异性好,是目前最精准的基因诊断方法。然而PCR方法操作起来较复杂,对仪器和人员要求比较高,不适合基层或现场快速诊断,因此在国内的推广速度并不是很快。 2000年日本学者Notomi在Nucleic Acids Res杂志上公开了一种新的基因诊断技术,即LAMP (Loop-mediated isothermal amplification),中文名为“环介导等温扩增反应”,受到了世卫
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