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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Rat IgG1 kappa monoclonal [RTK2071] - Isotype control
- 应用范围:
FC,IHC-Fr,IHC-P,IP,WB
- 宿主:
Rt
- 适应物种:
Rt
- 是否单克隆:
多克隆
- 保存条件:
4°C/-20°C
- 亚型:
IgG1
- 规格:
500ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
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