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文献和实验Studies of the Ubiquitin Proteasome System
of Small Interfering RNAs (siRNAs) to Reduce Expression of Ubiquitin‐Proteasome System Proteins in Cells Basic Protocol 13: Localizing Degradation to the Proteasome The NEDD8 Conjugation System Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1
until the solution becomes viscous and slightly clear. Do not allow the lysis reaction to proceed for more than 5 min. Add 350 μl Buffer N3 and invert the tube immediately but gently 4�6 times. To avoid localized precipitation, mix the solution gently
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
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