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文献和实验4 0.17% Glucose 1.0% Thallium Acetate 0.025% Penicillin 500u/ml Horse Serum
Cell Culture Media and Solutions
the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS
Preparation of Powdered Tissue Culture Media
reagents to the flask for each liter of media: 3.7 g sodium bicarbonate: 2 tubes of histidine solution (21 mg/ ml) 10 ml sodium pyruvate solution (100x) (GIBCO) 10 ml Penicillin/Streptomycin Solution (GIBCO) 5. Adjust the pH
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