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文献和实验Polyclonal and Monoclonal Antibodies
subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed with fusion 4 days later. OR III - Boost 3 days in a row with 15 ug Ag in PBS and proceed with fusion on 4th day. Cell Lines: 1) Myeloma; P3X63-Ag8.653. Origin: BALB/c, non
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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