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文献和实验of EzH2 in ∼2,000 cells. Initially, cultured PANC-1 cells were used to optimize protein electrophoresis and western blotting conditions. Gradient gel electrophoresis in combination with optimized antibody concentrations, and a sensitive chemiluminescent
有anti-phosphothronine 和anti-phosphotyrosine的抗体,PAb和MAb都有,但是没有anti-phosphoserine的抗体。 摘抄: Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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