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文献和实验Microbiology - Serial Dilution
. +900 mL Buf. +900 mL Buf. +900 mL Buf. Yield: 1000 mL 10-1 1000 mL 10-2 1000 mL 10-3 1000 mL 10-4 4. Since 100 mL of the 10-1 dilution is used to make the 10-2 dilution, only 900 mL remains for use. 5. The buffer used for dilution
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
Steve HahnProtein Dilution Buffer 5ml20 mM Tris pH7.9 100 microliters 1M Tris 7.9150 mM KCl 0.75 ml 1 M KCl1 mM DTT 50 microliters 0.1 M DTT10% glycerol 1 ml 50% glycerol50 micrograms/ml BSA 2.5 microliters 100 mg/ml BSA3.1 ml H2OOptional: add Brij
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