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文献和实验Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum
for 5 min at room temperature. 11. To elute nucleic acid from the column, centrifuge at 8,000 x g for 1 min. Retain flow-through containing the nucleic acid. Place column into a second 1.5 ml tube.
Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
by gel electrophoresis, although details are given for measuring DNA spectrophotometrically. The appendix lists alternative methods and larger scale purification protocols. Time scale Mini-preps involve an overnight growth step: Day 1(PM) Set
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