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文献和实验Vacuum Protocol: Purification of Viral Nucleic acid from Plasma or Serum
for 5 min at room temperature. 11. To elute nucleic acid from the column, centrifuge at 8,000 x g for 1 min. Retain flow-through containing the nucleic acid. Place column into a second 1.5 ml tube.
Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids
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