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Apogee流式细胞仪A50·循环微粒检测

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  • Apogee
  • Apogee A50
  • 2026年01月04日
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    科研产品,不能用于临床
    大部分流式细胞仪主要针对哺乳动物细胞而设计,但是细菌不同,它们的体积比哺乳类的细胞小三个数量级,因此产生的光学信号非常微弱。
    Apogee 公司的A50系列突破传统流式细胞仪,具有无法匹敌的灵敏度(<100nm)、光散射分辨率(20nm),能检测到比普通细菌小两个数量级的微生物,在微生物检测和循环微粒检测领域处于领先地位。仅用光散射,细菌、古生菌、循环微粒以及大部分病毒都能被精确检测。


    安全、简单、可靠

    · 专注——只从事分析型流式细胞仪的研制、开发
    · 专业——操作简单、结果可靠
    · 专长——聚焦经济型流式细胞仪,小巧、轻便

    轻便、小巧、灵活

    · 系统内部PC 配置Windows XP 操作系统,只需外接显示器、键盘、鼠标即可。
    · Apogee 开、关机方便,与普通电脑开关机无异,开机即可使用,不需预热时间。
    · 机身轻巧、方便移动。
    · 如需在室外使用,可选配带触屏装置的流式细胞仪。


    更高的质量、更好的技术

    ·灵敏度100nm

    ·分辨率20nm

    ·样品流速1.39-200ul/min多档速度可调

    ·快速的数据获取性能,可高达100,000 events/sec

    ·宽泛的样品检测范围,102-109cell/ml

    ·高自动化、强灵活性

    ·支持EP管、流式管检测, 鞘液为去离子水,没有任何后期耗材费用

    ·内置电脑和Windows操作系统 (只需要配置显示器、鼠标和键盘即可)

    ·内置注射泵喷嘴,无需担忧样本堵塞管路问题(可放心上样浑浊的环境样品等)

    ·可升级,支持多孔板测读

    ·病毒、古生菌、细菌 循环微粒 市场上的流式细胞仪通常用RNA或DNA染色的方法来检测体积较大的病毒,小体积病毒的散射光信号低下,难以捕捉到。而Apogee 在这领域的散射光和荧光检测处于领先地位,在科研领域让病毒、古生菌、细菌、循环微粒检测变得非常容易。

    ·食品和饮料工艺开发和监测(如益生菌,发酵)

    ·污水厂的监测和建模

    ·水质的快速评价

    ·快速细胞计数,快速DNA含量、细胞膜完整性、细胞内活性测定,等……

    ·遗传学研究(如古生菌、细菌细胞周期研究)

    ·海洋生物学(如浮游生物群落分析)

    ·病原体快速检测

    ·免疫学(如淋巴细胞表型分析)

    ·DNA分析

    ·精子分析

    ·生物量评估

    ·……

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    该产品被引用文献

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    相关实验
    • 细胞外囊泡(细胞微粒、外泌体)检测新趋势

      率 英国Apogee公司的A50-Micro突破传统流式细胞仪检测极限,优化的光学模块和优异的散射光检测能力使得A50-Micro具有无法匹敌的灵敏度(通过前向角散射光FC500只能勉强区分0.5μm和0.9μm的微珠,0.5μm以下的微珠则完全无法区分;Gallios和Influx在散射光检测能力方面有所提高,可以区分0.3μm和0.5μm的微珠,但0.3μm以下的微珠还是无法区分;而Apogee A50-Micro可以轻松地将0.14μm的微珠和0.3μm的微珠分成两个群(Fig

    • 610A 采用 CytoFLEX 流式细胞仪上的紫光侧向散射光,有利于进行亚微颗粒分析和计数

      背景和目标基于 FCM 的纳米颗粒(SMP)检测需要特殊的散射光参数设置。该参数的设置和标准化可以用微球作为 QC 工具实现。目标:采用流式细胞仪 (FCMr) 对 SMP 进行分析和计数。● 采用微球对最有用的散射参数(FSC 或 SSC)进行测定● 采用先前的标准化设置(1、2)对其标准化效果进行测试● 对比蓝光和紫光 SSC 的散射灵敏度和分辨率● 确认采用质控微球检测散射分辨率得到的增益值,是否会使检测细胞源微颗粒(MP ,亦称为 EV)具有较高灵敏度检测出现灵敏度增益值。● 测试体积

    • 流式细胞在血小板研究中的应用

      板体外活化→荧光染色(FITC、PE、PerCP三色荧光)→上机操作→分析数据。 荧光染色 BDIS抗体 FITC  PE  PerCP 对照管 PAC-1+RGDS  IgG1  CD61 试验管 PAC-1  CD62P  CD61 三色流式细胞分析法可以同时检测血小板反应性及其活化进程,能直接、同时检测多种血小板表面标志,使用全血、样本量少,操作简便快捷,将血小板人工活化降至最低。 (1)血小板微粒的测定 血小板微粒(PMPs)是血小板被激活后从质膜脱落进入外周循环的细小微粒

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