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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
HEPES, FREE ACID, ULTROL, 1M SOLN
- 供应商:
北京智杰方远科技
- CAS号:
******
- 保存条件:
详见说明
- 规格:
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文献和实验M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin.1M Hepes: 119.1 g Hepes + 500 ml dH2 O.Media A: 1X HBSS (10X:50ml) + 10 mM Hepes (1M: 5 ml) + 5 mM EDTA (0.5M: 5ml) + 2.5 % FBS (12.5 ml) in 500 ml.Media B: RPMI1640 470 ml + Gent
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
Introduction In our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. The cytoplasmic extracts may be prepared from cells which were treated in culture
Procedure i) Radioiodination of protein in solution *Reading a free booklet available from Amersham ("Guide to radioiodination techniques") is recommended. 1. In clear plastic tube, add 200µl of 0.1M phosphate buffer, pH 7 2. Add 5µl (500µCi
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