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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
IN Cell 3000 - Software
- 供应商:
北京智杰方远
- 保存条件:
详见产品说明
- 规格:
**
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文献和实验Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells
to label phosphorylated target proteins; images were captured with the INCell Analyzer 3000 and analyzed with the INCell Analyzer 3000 software. The first of these two assays monitors phosphorylation of ERK1/2, while the second assay monitors activation
buffer and add 250 µl of BSA (20 mg/ml in HBST buffer) to each well. Incubate for 1 hr at room temperature and then wash the wells with HBST buffer. This will block nonspecific protein-binding sites. Prepare the cell
Visualization of Bioluminescence
such as temperature control and agitation of samples are usually available at an extra cost. Most of the systems can be driven by computer with commercially available or customized software. Storage, display, and analysis of data involve the same or additional
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