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文献和实验CRISPR:细菌体内一串规律成簇的间隔短回文重复 (Clustered Regularly Interspaced Short Palindromic Repeats)DNA 序列,是大多数细菌及古细菌中一种获得性免疫系统。现在使用的 CRISPR/Cas 9 系统是由最简单的 type II CRISPR 改造而来,该系统由单链的 guide RNA 和有核酸内切酶活性的 Cas 9 蛋白构成。原理此系统的工作原理是 crRNA(CRISPR-derived RNA) 通过碱基
Anti-factor Xa (Anti-Xa) Assay
The anti-factor Xa (anti-Xa) assay is a functional assay that facilitates the measurement of antithrombin (AT)-catalyzed inhibition of factor Xa by unfractionated heparin (UFH) and direct inhibition of factor Xa by low-molecular-weight
Anti-2GP-I and Anti-Prothrombin Antibodies Generated by Phage Display
This chapter describes the construction and screening of a library of single-chain variable fragments (svFv) derived from patients with autoimmune disease. The methods cover the isolation of mononuclear cells from peripheral blood
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