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文献和实验Purification of Plasmid from 50 ml-culture
4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin
Purification of Plasmid from 50 ml-culture
. 4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
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