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文献和实验Isolation of micro-RNA (miRNA)
the sample in a microcentrifuge for 30 minutes for maximal recovery of the RNA. Spin the sample in a microcentrifuge at 13�14k rpm for 5 minutes Remove supernatant completely Wash pellet in 2× volume D of 75% ethanol�25% DEPC-treated
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
again. Centrifuge at maxi speed (13,000 x g) for 3 minutes at room temperature (22-25℃). The ETR Reagent should form a blue layer at bottom of the tube. 7. Transfer the top aqueous phase (cleared lysate) into a new 2 ml microtube and add 0.5 volume of absolute
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
-through. Then with the collection tube empty, centrifuge the spin cartridge at 10,000 x g for 2 min at room temperature to completely dry the HiBind® matrix. 11. Transfer the column to a clean 1.5 ml micro centrifuge tube (not supplied with kit) and elute the RNA with 30-50
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