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文献和实验-HCL PH 6.6, and that is better) Buffer PE 10 mM Tris-HCl pH 7.5 80% ethanol Buffer QBT equilibration buffer 750 mM NaCl 50 mM MOPS pH 7.0 15% isopropanol 0.15% triton X-100 Buffer QC wash buffer
of 10-15 ug DNA. If the expected yield is larger, divide the sample into the appropriate number of columns. 5) Add 300 ul of Binding Buffer (XP2) into the column. Centrifuge at 10,000 x g for 1 min at room temperature to wash the column. Discard
the collection tubes for next step. 6) Add 700 ul of SPW Wash buffer diluted with ethanol to the HiBind™ MicroElute™ DNA column and spin at 10,000 x g for 1 minute. Discard the flow-through and place the column back into same 2mL collection tube.
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