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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
(see recipe in unit 21.5 ) Rabbit pump (Rainin) with tubes 6000 to 8000 MWCO dialysis tubing
Western Blotting Using the SemiPhor Semi-Dry Transfer Unit
the unit to a suitable power supply. Turn the power supply to zero before turning on. Turn on the power supply and set to approximately 0.8 mA/cm2 of gel. 8 mm x 7 mm mini-gels may be run at 100 mA constant current. Larger gels must be limited to 0.8 mA/cm
Transformation of E. coli by Electroporation
ionic strength and a high resistance. The DNA may be purified by either precipitation or dialysis. Purifying DNA by Precipitation: 1. Add 5 to 10 μg of tRNA to a 20 μl ligation reaction. Add 22 μl 5M ammonium acetate. Mix well. 2. Add 100 μl
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