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文献和实验for PCR that vary in significant ways. The most significant components are the [Mg2+] and whatever is used to stabilize the enzyme. In theory [Mg2+] should be optimized for each primer/template combination. In practice this is seldom
; 72degC extension for 1 minute; 95degC denaturation for 4 seconds; 72degC extension for 1 minute; 95degC denaturation for 4 seconds; and 72degC extension for 1 minute), and then will link to a 4degC soak file until that program is aborted
for 1 minute), and then will link to a 4℃ soak file until that program is aborted. (It is possible to freeze the reactions at -20℃ after cycling, prior to the pooling step). 5. Briefly centrifuge the plate to reclaim condensation. Pool the four base specific
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