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文献和实验The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates
HIGH RESOLUTION GENETIC FOOTPRINTING
% acetic acid/0.5% Coomassie Brilliant Blue), destain (40% methanol/10% acetic acid) Bradford reagent for protein assays (Biorad) Sonicator SW28 rotor, ultracentrifuge ice, liquid nitrogen, -80°C freezer BUFFERS AND SOLUTIONS NB: Use autoclaved
to remove by dialysis; **** Phenol -containing detergents (for example Triton X-100 and NP-40) precipitate during Folin protein assay (but do not interfere with Bradford protein assay). N-Lauroylsarcosine Na (store at NT
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