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文献和实验Considerations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minutes.The Bradford is recommended for general use
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates
protocols are also described in the flyer that comes with the kit. Principle The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding
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