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文献和实验horseradish peroxidase an alternative to the more frequently used colloidal gold. This chapter describes a pre-embedding method for carbohydrate localization in which tissue sections are incubated with horseradish peroxidase-conjugated lectin
Western Blotting with Horseradish Peroxidase Conjugates
7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED
colored compound (chromophore), or light generated by a chemiluminescent reaction. The enzyme may itself be directly coupled to the probe (a direct label) or may be introduced by means of an antibody-enzyme or streptavidin-enzyme complex, which detects
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