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- 文献和实验
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- 抗体英文名：
Flow Cytometry Staining Buffer 600 mL
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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
Intracellular Immunofluorescent Staining for Flow Cytometry
. Incubate in the dark at room temperature for 20 minutes. Add 1ml of Permeabilization Buffer, centrifuge for 5 minutes and aspirate supernatant. Resuspend the cell pellet in 0.5ml of Flow Cytometry Staining Buffer. Analyze samples
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
PROCEDURE for preparation of activated platelets 1. Centrifuge tube of freshly drawn HEP or EDTA blood at 600 rpm (75xg) for 20 minutes.2. Remove all of platelet rich plasma (top layer) and place in clear 15 ml conical tube.3. Wash platelets
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