Flow Cytometry Fix Buffer (100

Intracellular Immunofluorescent Staining for Flow Cytometry

. Incubate in the dark at room temperature for 20 minutes. Add 1ml of Permeabilization Buffer, centrifuge for 5 minutes and aspirate supernatant. Resuspend the cell pellet in 0.5ml of Flow Cytometry Staining Buffer. Analyze samples

Flow Cytometry Analysis

Purpose Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based

Indirect flow cytometry (FACS) protocol

are difficult to resuspend. It is always useful to check the viability of the cells which should be around 95% not less than 90%. 2. Resuspend the cells to approximately 1-5 x 10 6 cells/ml in ice cold PBS, 10% FCS, 1% sodium azide. Use ice