Phospho-Drosophila p70 S6 Kina

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available

ERK-MAP Kinase Signaling in the Cytoplasm

. We describe two complementary methods for quantitatively measuring ERK activity toward the cytoplasmic p90 ribosomal S6 kinase (RSK). The first method is a straightforward immunoblot of endogenous ERK and RSK phosphoepitopes using phospho-specific antibodies. Infrared

Measurement of Phosphoinositide 3-Kinase Activity

only PtdIns as a substrate (e.g., mammalian PtdIns 3-kinase and yeast Vps34p) ( 1 ). Generally, PI 3 kinases are now regarded as an important intracellular signal upstream of a variety of biochemical (e.g., activation of Akt/protein kinase B and/or p70