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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
pSTBLUE-1 PERF. BLUNT GIGA CLONING KIT
- 规格：
40RXN
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文献和实验3’-A overhang to each end of the PCR product. Blunt cloning vectors and directional TOPO® cloning technologies are designed to clone PCR products produced by proofreading polymerases. Successful cloning depends upon using the correct polymerase with your cloning
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
Cloning via Restriction Digests
Steps for Cloning Prepare DNA. See Recovering Plasmids from your Stab Culture protocol if you need to prepare DNA from a bacterial stock. See PCR protocol if you are using PCR to generate an insert. Select
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