- ￥3996
- Abcam
- ab76911
- 2025年07月11日
- Ms
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD11c
- 宿主：
Ms
- 规格：
100ug
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文献和实验Polyclonal and Monoclonal Antibodies
Research, Inc. ), the latter being less hazardous than the first. 1) 1st injection: 15 - 50 ug Antigen in 200 ul adjuvant, injected subcutaneously. 2) 2nd injection: 2 weeks later; boost with 15 - 50 ug of Antigen in adjuvant. (If time permits
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Western Blotting with Monoclonal Ant
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10-15 seconds
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