质粒DNA小量提取与纯化试剂

Note 1: Once the kit is unpacked, store components at room temperature,except buffer CellR, which should be stored at 2-8℃. This will ensure the best performance, due to preserving activity of RNaseA included into the buffer. In case of occasional buffer ingredients precipitation, simply warm up in 37℃ water bath, until clarified.
Note 2: All solutions should be kept tightly closed to avoid evaporation and resulting components concentration changes.
Note 3: To obtain plasmid DNA of the highest purity it is important to follow carefully the protocol provided below.
Note 4: This kit is Chimerx Program exclusively produced for Andygene.
Kit Contents

Components	20 Reactions	100 Reactions
Buffer PX	800μL	4mL
Cell R buffer	5 mL	25 mL
LysisBlue buffer	4 mL	20 mL
Neutral-B buffer	7 mL	35 mL
PX buffer	10 mL	50 mL
Wash-PX buffer	13 mL	65 mL
Elution-PX buffer	1.5-2 mL	7.5-10 mL
DNA binding spin-columns	20	100

Description:
GeneMATRIX is synthetic, new generation DNA-and RNA-binding membrance, selectively binding nucleic acids to composite silica structures. Novel binding and washing buffers are developed to take full advantage of GeneMATRIX capacity, yielding biologically active, high-quality nucleic acids.
Matrix is conveniently prp-packed in ready-to-use spin-format. Unique chemical composition of the matrixes along with optimized construction of spin-columns improve the quality of final DNA or RNA preparation. To speed up and simplify isolation procedure, the key buffers are colour coded, which allows monitoring of complete solution mixing and makes purification procedure more reproducible.
As a result, we offer kits, containing matrixes and buffers that guarantee rapid, convenient safe and efficient isolation of ultrapure nucleic acids. Such as: restriction digestion, dephosphorylation, kinasing,ligation, protein-DNA interaction stuidies, sequencing, blotting, in vitro translation, cDNA synthesis, hybridization among others. Additional advantage is reproducibility of matrix performance, as component preparation is carried at Chimerx.
GeneMATRIX Plasmid Miniprep DNA Purification Kit is designed to isolate very high purity plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains. Plasmid DNA contaiminants such as: RNA, single-stranded DNA, enzymes/proteins, lipids endotoxins, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed from crude bacterial lysate. The membranes used for construction of the Plasmid Miniprep Kit is especially optimized toward removal of problematic inhibitors of restriction of DNA as well as non-specific endo-and exonuleases. Coloured lysis buffer helps both in monitoring cell solubilization peogress as well as simultaneous processing pf multiple samples. Optinized buffer is added to provide selective conditions for DNA binding to the GeneMATRIX during brief centrifugation, while contaminants pass thronughthe spin-column. Traces of contaminants remaining on the membrance are efficiently removed in two wash steps. High-quality DNA is then eluted in low salt buffer, e.g.:Tris-Hcl,TE or water. Izolated is ready for downstream application withall the need for ethanol precipition.