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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
First Strand cDNA Synthesis Kit
- 供应商：
北京智杰方远科技
- 保存条件：
详见说明
- 规格：
100 react
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文献和实验Protocol for First-strand cDNA Synthesis
℃for 10 minutes and then at 37℃for 60 minutes.Stop the reaction by heating at 70℃for 10 minutes. Chill on ice.NoteThe synthesized cDNA can be amplified by the PCR (see Protocols for PCR using Taq and Pfu DNA Polymerases) without intermediate phenol
of mRNA) or random hexamers; the kit used here contains both kinds); nucleotides for DNA synthesis (dNTPs); MgC2 and buffers required by the enzyme, which are all present in the 5x Reaction Mix. Materials * iScript cDNA Synthesis Kit (BIORAD
methylation analysis, PCR, and multiplex PCR. The purified total RNA is ready to use in any application, such as RT-PCR, real-time RT-PCR, differential display, cDNA synthesis, Northern, dot, and slot blot analyses, and microarray analysis. The kits form
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