MTT细胞增殖及细胞毒性检测试剂盒

MTT细胞增殖及细胞毒性检测试剂盒

MTT可以被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物formazan。在特定溶剂存在的情况下，可以被完 全溶解。然后通过酶标仪可以测定490nm波长附近的吸光度。细胞增殖越多越快，则吸光度越高；细胞毒性越大，则 吸光度越低
MTT细胞增殖及细胞毒性检测试剂盒
货号：M1020 规格：500T
保存：MTT溶液-20℃保存；Formazan溶解液室温或-20℃保存。


产品内容：
MTT溶液 5ml Formazan溶解液 60ml
产品简介： MTT可以被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物formazan。在特定溶剂存在的情况下，可以被完全溶解。然后通过酶标仪可以测定490nm波长附近的吸光度。细胞增殖越多越快，则吸光度越高；细胞毒性越大，则吸光度越低。
使用说明： 1.收集对数期细胞，调整细胞悬液浓度，分于96孔板，每孔180μl，3000-10000个细胞/孔。 2.置37℃、5%CO2温箱培养使细胞贴壁，培养6-24小时。 3.加入适当浓度的受试化合物，继续培养适当时间。 4.小心吸去上清，加入90μl新鲜培养液，再加入10ul MTT溶液，继续培养4 h。 5.然后吸掉上清，每孔加入110ul Formazan溶解液，置摇床上低速振荡10 min，使结晶物充分溶解。在酶联免疫检测仪490 nm处测量各孔的吸光值。 6.同时设置调零孔（培养基、MTT、 Formazan溶解液），对照孔（细胞、相同浓度的药物溶解介质、培养液、MTT、 Formazan溶解液），每组设定3复孔。
注意事项： 1.由于使用96孔板进行检测，如果细胞培养时间较长，一定要注意蒸发的问题。一方面，由于96孔板周围一圈最容易蒸发，可以采取弃用周围一圈的办法，改加PBS，水或培养液；另一方面，可以把96孔板置于靠近培养箱内水源的地方，以缓解蒸发。 2.MTT溶液为黄色需避光保存，长时间光照会导致失效。当颜色变为灰绿色时，请勿使用。Formazan溶解液结冻或产生沉淀时可以37℃水浴孵育以促进溶解，并且必须在全部溶解并混匀后使用。 3.为了您的安全和健康，请穿实验服并戴一次性手套操作。
相关产品：
12100 DMEM(H)
31800 RPMI Medium 1640
24800 通用细胞冻存液 Solarbio
P1020 1×PBS，PH7.2-7.4，0.01M
H1020 Hanks，含钙镁，含酚红
T1300 胰蛋白酶－EDTA消化液(0.25%)不含酚红
H1095 1M Hepes溶液(PH7.2-7.4，无菌)
P1400 青链霉素混合液(100×)
N8010-5Naphthalene acetic acid(NAA) α－萘乙酸5g
C8190-250Colchicine 秋水仙碱250mg

A8060-25Abscisic acid (ABA) 　脱落酸25mg
A8060-100Abscisic acid (ABA) 　脱落酸100mg
F8080-1Forchior fennron 氯吡苯脲 (促进植物细胞分裂)1g
X8040-5Zeatin 玉米素 (细胞分裂素，用于植物组织培养)5mg
T8110-50Trans-Zeatin (反玉米素 植物细胞分裂素)50mg
T8050-100Thidiazuron (TDZ) 噻苯隆100mg
C8790-100Chlormequat 矮壮素100g

K8010-250Kinetin 激动素250mg
A8170-16-Benzyl Aminopurine 6-苄氨基喋呤1g

公司简介：
北京索莱宝科技有限公司始创于2000年，是一家集产品研发、生产、销售、服务于一体的大型高科技生物企业。 公司总部位于北京，拥有1200平米研发生产中心，公司提供的产品近万种，超过5000种常备现货，涵盖分子生物学、细胞生物学、免疫学、生物医学等相关领域，并不断推陈新出扩充种类，为用户提供优秀，专业的产品。



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