可吸收糖类和膳食纤维检测试剂盒 Available Car

产品货号：K-ACHDF

品牌：Megazyme

产品品名：可吸收糖类和膳食纤维检测试剂盒

英文品名：Available Carbohydrates/Dietary Fibre Assay Kit

规格型号：100 assays of each component

An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products.

INTRODUCTION:
A need for information on the carbohydrate composition of foods for diabetics prompted McCance and Lawrence1 to attempt to measure this to gain results that would be of biological significance. They divided the carbohydrates in foods into two broad groups, ‘available’ and ‘unavailable’. The available carbohydrates, that is sugar plus starch, were defined as those that are digested and absorbed by the human small intestine and which are glucogenic. The unavailable carbohydrates were defined as those that are not digested by the endogenous secretions of the human digestive tract. These are now generally referred to as dietary fibre.
The concept of ‘available’ and ‘unavailable’ carbohydrates drew attention to the fact that some carbohydrates are not digested and absorbed in the small intestine but rather reach the large bowel where they are fermented. In the FAO/WHO report on “Carbohydrates in Human Nutrition”2, it was stated that these terms can be misleading since the so-called ‘unavailable’ carbohydrate can, in fact, provide energy to the body through fermentation. This committee recommended use of the term ‘glycemic’ (i.e. providing carbohydrate for metabolism) to replace ‘available’. However, this term has not gained widespread acceptance, possibly because there is some confusion with the well recognised term ‘glycemic index’. For this reason, we have decided to continue using the term ‘available carbohydrates’ for carbohydrates that are digested and absorbed by the human small intestine; these include D-glucose,
D-fructose, sucrose, maltodextrins, non-resistant starch and the
D-glucose component of lactose; all measured as D-glucose plus
D-fructose following enzymic hydrolysis.
Dietary fibre is a mixture of complex organic substances including a range of hydrophilic compounds such as soluble and insoluble polysaccharides and non-digestable oligosaccharides as well as non-swellable, more or less hydrophobic compounds such as cutins, suberins and lignins. The procedures for the determination of total dietary fibre as outlined in this booklet are based on the methods of Lee et al.3 and Prosky et al.4 (AOAC Official Method 991.43, AACC Method 32-07). These methods do not measure non-digestible oligosaccharides, as these remain soluble in the ethanolic solution used to precipitate high molecular weight dietary fibre components.

KITS:
Kits suitable for performing 100 assays of each are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 7.6) plus sodium azide(0.02% w/v) as a preservative.Stable for > 3 years at 4°C.
Bottle 2: NADP+ plus ATP.Stable for> 5 years at -20°C.
Bottle 3: Thermostable α-amylase (6 mL, 3,000 U/mL)(no. E-BLAAM).Stable for > 3 years at 4°C.
Bottle 4: Protease (10 mL, ~ 350 U/mL)(no. E-BSPRT).Stable for > 3 years at -20°C.
Bottle 5: Amyloglucosidase (20 mL, ~ 3,300 U/mL)(no. E-AMGDF).Stable for > 3 years at 4°C.
Bottle 6: Sucrase plus β-galactosidase, freeze-dried powder.Stable for > 3 years at -20°C.
Bottle 7: Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 2.25 mL.Stable for> 3 years at 4°C.
Bottle 8: Phosphoglucose isomerase suspension (2.25 mL). Stable for > 3 years at 4°C.
Bottle 9: D-Glucose plus D-fructose standard solution(5 mL, 0.2 mg/mL of each sugar).Stable for > 3 years at room temperature.

EQUIPMENT (RECOMMENDED):
1. Duran? bottles (250 mL volume).
2. Fritted crucible, Corning No. 36060 Büchner, fritted disk, Pyrex
60 mL, pore size, coarse, ASTM 40-60 μm, or equivalent. Prepare as follows:
a. Ash overnight at 525°C in muffle furnace.
b. Remove Celite and ash material by using a vacuum.
c. Soak in 2% (v/v) Micro cleaning solution (reagent 6) at room temperature for 1 h.
d. Rinse crucibles with water and then deionised water.
e. For final rinse, use 15 mL acetone and air dry.
f. Add approximately 1.0 g Celite to dried crucibles and dry at 130°C to constant weight.
g. Cool crucible in desiccator for approximately 1 h and record weight of crucible containing Celite.
3. Filtering flask, heavy-walled, with 1-L side arm.
4. Rubber ring adaptors for use on filtering flasks.
5. Vacuum source: vacuum pump or aspirator with regulator capable of regulating vacuum.
6. Water bath, shaking, large-capacity (20-24 L) with covers; capable of maintaining temperature of 100°C; equipped with automatic timers for on-off operation.
7. Balance, 0.1 mg accuracy.
8. Ovens, two, mechanical convection, set at 103 ± 2°C and
130 ± 3°C.
9. Desiccator, airtight, with SiO2 or equivalent desiccant. Desiccant dried biweekly overnight in 130°C oven.
10. pH meter.
11. Pipettors and tips, 50-200 μL and 5 mL capacity.
12. Dispensers
a. 15 ± 0.5 mL for 78% (v/v) EtOH, 95% ethanol, and acetone.
b. 40 ± 0.5 mL for buffer.
13. Measuring cylinder, 500 mL.
14. Magnetic stirrers and stirring bars.
15. Rubber spatulas.
16. Muffle furnace, 525 ± 5°C.
17. Microfuge, capable of 12,000 g.

温馨提示：需要详细的产品使用说明书，请联系甄准生物客服人员，我们将竭诚为您服务。

甄准，甄心倾听您每一个标准！