膳食纤维总量检测试剂盒Total Dietary Fibre

产品货号：K-TDFR

品牌：Megazyme

产品品名：膳食纤维总量检测试剂盒

英文品名：Total Dietary Fibre Assay Kit

规格型号：200 assays per kit

AOAC Method 985.29, AOAC Method 991.42, AOAC Method 993.19, AOAC Method 991.43, AACC Method 32.07.01, AACC Method 32.21.01 and AACC Method 32.05.01. For the measurement of total, soluble and insoluble dietary fibre according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81 No. 1, 1998.

INTRODUCTION:

Dietary fibre is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination of total dietary fibre as outlined in this booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fibre Kit can also be used in other dietary fibre analytical methods such as AACC Method 32-21.01 and AACC method 32-06.01.

KITS:

KKits with reagents for 200 assays are available from Megazyme and contain the full assay method plus:
Bottle 1: Thermostable α-amylase (20 mL, ~ 3,000 U/mL (Ceralpha method); ~ 10,000 U/mL on soluble starch) (Megazyme cat. no. E-BLAAM).
Bottle 2: Purified protease (20 mL, 50 mg/mL; ~ 350 tyrosine Units/mL) (Megazyme cat. no. E-BSPRT).
Bottle 3: x 2 Purified amyloglucosidase (20 mL, 3300 U/mL on soluble starch) (Megazyme cat. no. E-AMGDF).
Celite?545, acid washed in 100 g or 500 g packages is available separately (cat. no. G-CEL100, or cat. no. G-CEL500).

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