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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
Merck
- 库存:
1
- 英文名:
ProteoExtract Protein Precipitation Kit
- 保存条件:
15-30°c
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文献和实验Acetone precipitation of protein
volume of acetone to -20°C. 2. Place protein sample in acetone-compatible tube, such as polypropylene and able to hold six times the sample. Screw cap tubes may help minimize sample losses. 3. Add six times the sample volume of cold (-20°C) acetone
Protein Precipitation Protocols
TCA-DOC For precipitation of very low protein concentration 1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent). 2) Vortex and let sit for 30min at 4ºC. 3) Add 1/10 of Trichloroacetic acid (TCA) 100
To concentrate proteins for analysis by SDS PAGE:If a small amount of protein is to be precipitated (less than a few micrograms),add Insulin as a carrier protein (10 micrograms of Sigma insulin,I-5500,per sample works well).1.Add an equal
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