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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
QuantiTect Probe PCR Kit (200)
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文献和实验using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
into the well according to the following volumes: 800 ul for soybean or similar sized seeds 600 ul for cotton or similar sized seeds 200 ul for canola, sorghum, wheat, or similar sized seeds 100ul for arabidopsis or similar sized
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
like to increase the concentration of cDNA to 200 ng/ul but am wondering if this can cause any problems. I use SYBR green. Also, my water blank with reference gene(18S) has a CT value of 32.0. I have run this with DEPC water, and two different bottles
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