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EpiTect Fast LyseAll Bisulfite Kit (50)
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文献和实验Mag-Binds Plant DNA Kit Magnetic Fast Protocol
preset at 65-70°C 4. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000) 实验步骤 1. Collect plant tissue (start with 50mg for fresh sample and 10mg for dried samples) in a 1.2 ml 96 well plate
by adding 55 mg of hydroquinone to 50 ml of water. Add 520 ml freshly prepared 3M Sodium bisulfite (Sigma S-8890), prepared by adding 1.88 gm of sodium bisulfite per 5 ml of H2O, and adjusting pH to 5.0 with NaOH. Assure that reagents are mixed with DNA
Bisulfite sequencing of very small samples
L of 10 mM hydroquionone and 255 µL of 40.5% Sodium bisulfite Incubate at 50 °C for 4-6 hours with gentle mixing (e.g. Eppendorf Thermomixer at 400 rpm), cover with aluminum foil to protect samples against light (note 7). Stop
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