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- 英文名：
Stainless Steel Beads 5 mm (200)
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文献和实验Yeast mini whole cell extract using glass beads for western analysis
in 20 ml cold extraction buffer (containing DTT and protease inhibitors).Keep everything cold from this point on. 3.Resuspend cells in 600 microliters extraction buffer and transfer to 13 x100 mm glass test tubes on ice.Add 400 microliters glass beads
Preparation of cell lysates from yeast using glass beads vortexing
Preparation of cell lysates from yeast using glass beads vortexing Equipment and reagents Lysis buffer 50 mM Tris-HCl pH 8.0 1% DMSO 50-200 mM NaCl* 1 mM
Yeast mini whole cell extract using glass beads for western analysis
from this point on. 3. Resuspend cells in 600 microliters extraction buffer and transfer to 13 x 100 mm glass test tubes on ice. Add 400 microliters glass beads. 4. In the cold room, vortex for 1 min on ice and keep on ice for at least 1 min
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