AllStars Neg. Control siRNA (2

siRNA设计指南

of controls to ensure the validity of the data. The editors of Nature Cell Biology have recommended several controls (2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant

siRNA Design Guidelines

(2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant sequence homology to the genome . To design a negative control siRNA, scramble the nucleotide sequence of the gene-specific

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

for the siRNA targeted gene compared to the respective control(s).Part III: Detection of pRNAs by 5'biotin EF52 pull-down assay 1.A minimum of ~4.0×10 6 cells are required/sample. Twenty-four hours following the plating (~70% confluent) the cultures