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- 英文名：
NeXtal Stock Jeffamine M-600 (200)
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
PREPARATION OF STOCK SOLUTIONS FOR FISH
, this results in solution of 0.2 M Tris-HCl and 0.02 % NaN3 -dissolve 2 mg of propidiumiodide (PI) in 2 ml of the above mentioned Tris-buffer, this gives a PI stock of 1 mg/ml -make aliquots of this PI stock of 10 µl and store at -20°C -put 0.69 g of di-azo
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