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- 英文名：
Protease Resuspension Buffer (6/15),KG
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
for one minute between mixes. o o o o o o o o o Spin @ 500rpm for 15 seconds. o Add 250µl of FA lysis buffer w/ protease inhibitors. o Stack Transfer - Transfer lysate to new tube 1. Label and open new tubes. 2. Wipe outside of old tube with ethanol
Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
. 7)Spin 15 seconds at 8,000 rpm. 8)Wash 3x with ~600 μl of resuspension buffer 9)Remove supernatant and resuspend in appropriate volume resuspension buffer.(You can add this directly to SDS load buffer for running on a gel.)
volume will be ~550 μl)and put into 4 eppendorfs.5)Add 300 μl of resuspension buffer and 75 μl of E.Coli.GST Lysate to each tube.6)Incubate for 30 min while rocking in cold room.7)Spin 15 seconds at 8,000 rpm.8)Wash 3x with ~600 μl of resuspension buffer9)Remove
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