AssayPro—Human PAI-1 ELISA Kit

Introduction
Type I plasminogen activator inhibitor (PAI-1) is a 43 kDa serpin family member that inhibits tissue- and urokinase-type plasminogen activators (t-PA, u-PA). This protein appears to be an important regulator of plasminogen activation by t-PA and extracellular proteolysis by u-PA (1, 2, 3). The plasminogen activator proteolytic enzyme systems are important not only for fibrinolysis but also for extracellular matrix remodeling and have been implicated in a number of normal and pathological processes including angiogenesis, ovulation and embryogenesis, thrombotic and hemorrhagic disorders, connective tissue diseases, neoplasm, and sepsis (4, 5). PAI-1 is a prognosticator in breast cancer (6), gastric cancer (7), various forms of lung cancer, (8) and cervical cancer (9).

【介绍】
纤溶酶原激活剂抑制物（PAI-1）是一种分子量为43KDa丝氨酸蛋白酶抑制物家族成员，主要作用是抑制组织型和尿激酶型纤溶酶原活化剂（t-PA，uPA）。PAI-1在调节t-PA活化纤溶酶原和uPA在细胞外间质的蛋白水解有重要作用。纤溶酶原激活剂的蛋白水解酶系统不仅对纤溶是重要的，对下面过程也是很重要的：即细胞外间质的再生；参与许多正常和病理的过程如血管的生成、排卵、胚胎的形成、血栓和出血的疾患；结缔组织病变，瘤和败血症等。
PAI-1是乳房癌、胃癌、各种肺癌和子宫颈癌的一个预后指标。


Principle of the Assay
The AssayMax Human PAI-1 ELISA kit is designed for detection of PAI-1 in human plasma, serum, tissue extracts, saliva, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures PAI-1 in less than 4 hours. A polyclonal antibody specific for PAI-1 has been pre-coated onto a microplate. PAI-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for PAI-1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.