AssayPro—Human Ferritin ELISA

Introduction
Ferritin is an iron storage protein. It consists of 24 subunits with combined molecular weight of 474,000 Da. Serum ferritin level is related to body iron stores and is influenced by several diseases. High serum ferritin levels associate with iron overload [1], diabetes mellitus [2], Adult-onset Still disease (AOSD) [3], excessive macrophage activation [4], and alcohol intake [5]. On the other hand, a low level of ferritin is an indication of iron deficiency anemia [6].

Principle of the Assay
The AssayMax Human Ferritin ELISA kit is designed for detection of human ferritin in plasma, serum, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures ferritin in less than 4 hours. A polyclonal antibody specific for ferritin has been pre-coated onto a microplate. Ferritin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for ferritin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.