AssayPro—Human C-Reactive Prot

Introduction
C-Reactive Protein (CRP) is a liver protein composed of five identical non-glycosylated subunits, with a total molecular weight of 105 kDa. CRP has a variety of powerful effects related to immunology, inflammation, and coagulation. As a marker of low-level inflammation, CRP appears to predict future cardiovascular disease events among apparently healthy individuals. High plasma concentration of CRP was associated with increased risk of stroke, myocardial infarction, and peripheral vascular disease (1-3). CRP has also been associated with increased risks of fatal coronary events among high-risk male smokers and incident coronary disease among the elderly (4, 5). Studies have established the prognostic usefulness of CRP in the setting of angina (6). Originally used as a marker of acute inflammation, CRP has become a leading candidate as the measure of choice for estimating the inflammatory component of cardiovascular disease risk.

Principle of the Assay
The AssayMax Human C-Reactive Protein ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human CRP in plasma, serum, saliva, milk, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures CRP in less than 4 hours. A murine antibody specific for CRP has been pre-coated onto a 96-well microplate with removable strips. CRP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for CRP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.